FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413270
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG4950.037.9835323
CTACTCG4850.036.8283652
CGGAGGT6000.034.4686247
CCGAGTA5750.031.0639232
GCTACTC12850.030.7267571
CGAGTAG5350.029.8720953
GGCGGAG8500.029.3081575
CTCGGGA7050.028.668895
ACTCGGG6750.027.854594
GCTGGGA40750.027.79799
TCGGGAG7800.027.72016
TTGGGAG32100.027.0893296
AGGCGGA9650.026.7901974
GGAGGTT22600.026.205038
GCGACTA909.517562E-426.1125958
CCCGAGT7450.025.8683151
GGGAGGC40300.025.775668
GGAGGCT34650.025.502179
AGTAGCT20650.025.2658825
TAGCTGG23400.024.7065337
Produced by FastQC (version 0.11.5)