FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216427
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA750.0026562647201.30794225-229
AAGGGCG6355.919675E-574.21183245
CCGTCAA3950.003170711263.998257240-244
CGAGTAG11700.051.5538143
TACTCGG13900.051.5052833
CCGAGTA12600.050.5557752
AGTAGCT47500.050.437265
GACGGAC4850.00816094350.400112235-239
CTACTCG14150.049.7985232
GCTGGGA109950.048.4973759
TTGGGAG82050.048.158016
GAGTAGC29650.047.9113854
GCTACTC33050.047.0761031
AAGTGCT69600.045.6799855
GTAGCTG50700.045.5832186
AAAGTGC70400.044.5210084
GGAGGCT88150.044.3132139
TAGCTGG52900.044.2195937
CCCAAAG90000.043.844831
AGTGCTG72900.043.454816
Produced by FastQC (version 0.11.5)