FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037172
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA1702.0172403E-4128.03375330-339
AGCGACT5850.001168210674.25397330-339
AGTAGCT63300.069.097615
CGAGTAG19350.066.35173
GTAGCTG67250.064.7650456
CGTTGCG1351.227984E-464.07743260-269
TAGCTGG73250.061.0534867
GAGTAGC45800.059.687214
AAGTGCT71150.059.488235
GCTGGGA145800.057.5545779
CCGAGTA22050.056.302772
TTGGGAG89650.052.146016
AAAGTGC82800.051.563364
AGTGCTG81750.050.948186
CCAAAGT90300.050.9097332
CCCAAAG101350.050.489541
CAAAGTG87500.050.057393
GTACGCC2600.008721868549.56747260-269
CCCGAGT26200.046.7147061
TACTACG3300.001749853846.64683340-345
Produced by FastQC (version 0.11.5)