FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037172
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA750.005981502153.47314225-229
TAGCGCG1200.001381795975.44968245
AGTAGCT61850.072.0489045
CGAGTAG17600.067.14113
GTAGCTG67700.066.25736
GAGTAGC42450.065.783024
AAGTGCT79800.062.104435
TGCGATA3050.003663306161.712704240-244
TAGCTGG74700.061.3833277
GCTGGGA153700.060.2779969
GCTACTC43950.060.1957551
CTACTCG18200.060.0813182
CCGAGTA20200.059.9533922
TTGGGAG103700.059.242786
CCAAAGT91350.057.9204442
AAAGTGC84250.057.4285434
TACTCGG18900.057.2349173
AGTGCTG85900.057.1467066
CAAAGTG89700.056.69293
GGAGGCT119350.055.7078829
Produced by FastQC (version 0.11.5)