FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053393
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC2300.007143788144.58249340-345
ACTATCG2052.3500605E-4123.20929250-259
TTACGGC3300.001133094282.94878260-269
AGTAGCT64500.066.4939045
GTAGCTG66350.063.2684676
ATCGGCG3209.231371E-961.544594270-279
TAGGCGT4450.060.71993310-319
GCTGGGA145000.058.8219579
GAGTAGC44450.058.5474054
TAGCTGG75600.058.25557
CGAGTAG19150.057.9701543
AAGTGCT73800.057.2104075
CGTATTC4450.00530081356.21001340-345
CTACTCG14700.054.0839162
CCGAGTA21700.052.5792
CGAGTAT3004.634785E-952.168606330-339
AAAGTGC80950.051.4078454
TTGGGAG90450.051.0385366
TACTCGG15500.050.878443
AGTGCTG84450.049.708266
Produced by FastQC (version 0.11.5)