FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053393
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACA3750.001888961672.92632245
CGAGTAG17100.070.514773
AGTAGCT63400.069.243815
CTACTCG19900.067.652482
CCGAGTA18550.066.5805742
GAGTAGC41050.065.449834
TACTCGG20900.064.9756553
GTAGCTG67850.063.666126
GCTACTC43400.062.8530731
AAGTGCT80700.062.3058175
GCTGGGA157200.060.2429859
TAGCTGG76600.058.834957
AAAGTGC86350.057.6867644
TTGGGAG101700.056.6913956
AGTGCTG88900.055.701966
CCAAAGT92500.055.6232222
GGAGGCT111350.055.2450079
TCGGGAG26150.055.0636866
GCACTTT79100.054.540781
CAAAGTG92450.054.32373
Produced by FastQC (version 0.11.5)