FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422869
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1006.9849193E-1042.3513641
CTAGCCG953.7578004E-529.679785
TACTCGG3500.028.195793
CCGAGTA4300.026.244892
CTACTCG3650.024.4772552
CGAGTAG3800.023.4964923
TAGGTCG1000.001760768923.496495
TTGGGAG25600.022.578666
CGTGTCG1050.002329865522.3914762
GCTGGGA35050.022.2563089
GGAGGCT27350.021.993069
TAGGCGG1303.1642374E-421.689075
GGGAGGC28250.021.625098
CGGAGGT5650.021.625097
GGCGGAG6350.021.4613635
GGGGGTA5500.021.3604479
GAGTAGC10850.021.2226374
TAACCGG1554.245859E-521.2226376
CGGGTTC4250.021.0086299
TAGCTGG21250.020.5663417
Produced by FastQC (version 0.11.5)