FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422869
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4000.036.434152
TACTCGG4000.036.43343
CGAGTAG4150.033.9837383
CCGAGTA4450.032.74982
CTCGCGT1052.0628977E-631.340564
TCGCGTT804.7759776E-429.3817735
TAGCGAG856.8090996E-427.6534354
TTGGGAG29750.027.4954136
GCTACTC10800.027.4258211
CGGAGGT6850.026.7625337
CCCGAGT6750.026.4680941
GGGAGGC34900.026.1294028
GCTGGGA36800.026.0576679
GGAGGCT33500.025.958539
ACTCGCG950.001303250124.7425483
GTCGGTT950.001304034324.7399928
CTCGGGA6800.024.1967565
GGAGGTT20850.024.1229788
GAGTAGC10850.023.830384
CGCCGTA1000.001758147723.50250894
Produced by FastQC (version 0.11.5)