FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628563
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5050.039.085093
CTACTCG5300.037.262012
GCTGGGA60300.034.8372159
CCCAAAG46250.034.2912331
AGTAGCT27350.034.1939245
TAGCTGG31200.033.4388667
CGAGTAG6200.033.351413
GCTACTC15600.032.580981
GTAGCTG30150.031.9535986
AAAGTGC37300.031.7500864
TTGGGAG40650.031.676946
GTGCTGG38400.030.9629617
GAGTAGC17950.030.8937594
CCGGTAG2000.030.5468628
CCAAAGT44650.030.1187822
AAGTGCT39600.030.024695
CCCGAGT8300.029.4842591
CCGAGTA7400.029.2293362
AGTGCTG40450.028.9290436
CAAAGTG43400.028.6952063
Produced by FastQC (version 0.11.5)