FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044211
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT3950.001176243282.16684300-309
CAACGTA3552.503839E-880.4596340-345
AGTAGCT72150.072.6973345
CGAGTAG19350.068.887753
GTAGCTG78100.067.555866
GAGTAGC50000.065.098924
CCGAGTA21100.064.382432
TAGCTGG83900.064.141977
AAGTGCT81850.064.006275
GCTGGGA167400.062.2582249
CTACTCG19150.060.896842
TACTCGG20000.059.829013
GCTACTC44750.058.121081
AGTGCTG92100.056.8155636
AAAGTGC91500.056.510544
TTGGGAG104000.055.739466
CCAAAGT100150.054.690952
TCGGGAG25700.054.52056
CAAGTAG32900.053.1419833
CAAAGTG99850.052.9024773
Produced by FastQC (version 0.11.5)