FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044211
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG2804.6814057E-6123.73351245
CGAGTAG20550.076.615973
AGTAGCT70300.075.945265
GAGTAGC48600.072.343514
GTAGCTG74500.071.578456
GCGTCAA3650.002078714871.18914245
CCGAGTA22600.069.6674352
CTACTCG21850.068.26622
AAGTGCT86250.068.008635
TACTCGG22850.066.572723
GCTACTC49500.065.7691
TTGGGAG114100.065.202376
TAGCTGG83500.065.139267
GCTGGGA173900.064.970029
AAAGTGC91550.062.649114
CGGCACG4150.003457922362.612133245
CCAAAGT98900.061.7048032
AGTGCTG94400.061.316966
GCACTTT90350.060.076541
CAAAGTG98400.059.3104553
Produced by FastQC (version 0.11.5)