FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466531
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5900.038.2333563
GCTGGGA62500.037.1449789
CTACTCG6150.035.1722452
AGTAGCT29600.035.0876055
CGAGTAG7000.034.9106983
CCCAAAG43150.034.6738131
GCTACTC14250.034.3379061
TAGCTGG32750.034.2957087
AAAGTGC34900.033.6641584
CCCGAGT8250.033.6475451
GTAGCTG30750.033.622566
AAGTGCT36650.033.4672245
TTGGGAG42800.033.160146
GAGTAGC18150.032.6247444
AGTGCTG38700.032.1801536
CCAAAGT37950.032.0926322
CAAAGTG39100.031.0095963
GTGCTGG40750.030.6765967
CCGAGTA8250.030.2092062
CGGAGGT10550.028.5089137
Produced by FastQC (version 0.11.5)