FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466531
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6500.047.7318082
CGAGTAG6150.046.626413
GCTACTC17550.043.6612931
TACTCGG7650.042.3999143
CCGAGTA6950.041.2593462
TTGGGAG45700.040.937176
AAAGTGC37400.040.471824
CCCAAAG49050.039.869471
GCTGGGA67950.039.4284639
GAGTAGC17800.039.3490944
CAAAGTG42550.038.777953
AAGTGCT39000.038.6909075
CCAAAGT45200.037.9604842
AGTAGCT29750.037.29015
AGTGCTG41250.037.1487776
GGAGGCT53800.036.43169
TCGGGAG10150.036.1226356
GTGCTGG43450.035.8065647
ACTCGGG8800.035.789914
TAGCTGG31300.035.7402087
Produced by FastQC (version 0.11.5)