FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796168
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1309.1895345E-932.54150894
CTACTCG6550.026.555432
GTCGCCG3350.025.25609694
CTCGGGA6350.025.170415
TACTCGG6750.025.0721533
GAGTAGC14150.024.9166684
CGAGTAG5600.024.3446123
CCGGGTT4450.024.2948448
GCTGGGA47700.023.256269
CCCAAAG39150.023.1761741
GCCCGTA1050.002334812222.383459
AGTAGCT24650.022.1220915
TCGGGAG8800.021.900566
CCGAGTA6050.021.7568262
TCGCCGT3251.8189894E-1221.69433894
TAGCTGG26850.021.357687
GTAGCTG26600.020.8523226
TTGGGAG33100.020.5917876
GGGAGGC38600.020.5800158
ACTCGGG9200.019.9279174
Produced by FastQC (version 0.11.5)