FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946490
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6100.034.6759833
CCGAGTA6600.032.7617722
CTACTCG4900.030.697812
GGAGGCT39550.030.4245939
TACTCGG4800.030.3575363
CCCGAGT7850.029.9411541
CGCCGTA1751.72804E-1029.54413294
GGGAGGC42450.028.1246668
CGGAGGT7700.028.0800887
GCTACTC13750.028.033631
GCTGGGA45600.027.9341729
AACCGCG700.00883343926.8601255
GAGTAGC14950.026.410964
AGTAGCT24950.026.1872795
CTCGCGT909.515052E-426.114014
TTGGGAG37250.025.741586
TGGGAGG45600.024.9449067
AGGCGGA10600.024.8329454
CTCGGGA6900.024.5244625
GTAGCTG26450.024.1682156
Produced by FastQC (version 0.11.5)