FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843430
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1400.040.3250351
CCGAACG753.2580548E-431.3639161
CGAGTAG5550.027.9432163
TACTCGG4650.027.2876593
CGGAGGT7400.026.673077
GCTGGGA47000.026.1974419
CCCGAGT7350.025.6031951
AGTAGCT26000.024.9437165
TAGCTGG28350.024.202227
GAGTAGC15800.024.0925834
TTGGGAG33450.023.7435686
CCGAGTA6500.023.1451552
GGCGGAG9600.023.008175
CTACTCG4550.022.7318482
CCCAAAG39100.022.6205251
GTAGCTG27950.022.5308956
AGGCGGA9000.022.4533624
GGGAGGC41350.022.1622858
GCGGAGG8450.021.690196
GGAGGTT22600.021.418268
Produced by FastQC (version 0.11.5)