FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843430
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG555.2833668E-542.7296079
CTCGCGT1256.2664185E-933.844234
CTACTCG5700.031.3389032
CGAGTAG5400.030.466773
CCGAGTA5800.029.9880892
TACTCGG5650.028.2867223
TTGGGAG38600.028.2507466
GCTACTC15550.028.1158051
CCCGAGT7400.027.9523891
GCTGGGA49100.027.6654629
TCGCGTT856.8135984E-427.6505155
GGCGGAG10550.027.6243065
CGGAGGT8850.027.6163567
GGAGGCT41800.027.324469
TAACGCG350.008840715526.85601234-35
GAGTAGC15150.026.3729324
GGGAGGC46500.026.1794648
CTCGGGA7050.026.003255
AGGCGGA10850.025.9940344
TGGGAGG49150.024.7675237
Produced by FastQC (version 0.11.5)