FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248076
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5400.049.621682
TACTCGG5800.047.8194663
CGAGTAG5050.046.5435333
GCTACTC14700.043.8140031
CCGAGTA5900.042.2293362
TTGGGAG40750.036.56656
CCCAAAG38650.035.395921
GAGTAGC15150.035.373084
GGAGGCT44300.035.120189
GCTGGGA57300.035.0272189
CCCGAGT7750.034.5766451
CCGCGTA352.1868458E-433.56924434-35
GGGAGGC50550.033.28858
CTCGGGA8650.033.1508335
AAAGTGC33100.033.0909044
AGTAGCT26200.033.013935
CGGAGGT10400.032.9930087
GTAGCTG26500.032.815396
TCGGGAG9600.032.806156
TAGCTGG26750.032.6829727
Produced by FastQC (version 0.11.5)