FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784859
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6700.037.1756023
CCGAGTA7150.036.1842272
GAGTAGC18650.031.750364
CTACTCG5650.031.6371782
GCTGGGA57000.031.5777289
CCCGAGT9350.031.2099111
TACTCGG5750.031.0579453
CCCAAAG41900.030.4416181
AGTAGCT28500.030.1761055
TTGGGAG38900.029.3571326
GTAGCTG29750.029.2241386
GCTACTC15550.029.0571671
CGTCGCG1154.202291E-628.6069
TAGCTGG32250.027.6873177
GGAGGCT42650.027.437049
GGCGGAG10000.026.787485
GTGCTGG36750.026.0873417
AAAGTGC36450.025.9152554
CCAAAGT40300.025.9125752
GCGACTA551.0181856E-425.63947560-61
Produced by FastQC (version 0.11.5)