FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728934
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1150.049.1146351
TACTCGG4100.029.8016363
CGGCCTA1751.72804E-1029.5396428
CGGAGGT7500.026.9437357
GCTATCG909.440809E-426.1489941
CGAGTAG5150.024.6380963
CTACTCG4850.024.2428252
CCGAGTA6100.022.3590372
CGTACTA1303.1470924E-421.7066522
GCTGGGA41450.021.6550629
TAGCTGG24900.021.1382647
GGGAGGC32450.020.9992548
TTGGGAG29250.020.725956
GGCGGAG7050.020.664425
CTCGTAT15550.020.5508294
GAGTAGC14450.020.1638974
GCGGAGG8550.019.7873216
AGTAGCT22100.019.7761295
AGGCGGA6950.019.6093794
GTAGCTG24200.019.2251826
Produced by FastQC (version 0.11.5)