FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728934
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA804.642061E-947.00147694
ACGCGTC250.001703320237.596762-63
TACTCGG5100.031.3406333
CTACTCG4850.031.0175342
CCGAGTA5400.029.5994872
TCGCCGT2450.026.85798594
GCTACTC13600.025.5813641
CCCGAGT6800.025.5813641
GGAGGCT34450.025.3767039
GCTGGGA42750.025.0674519
CTCGGGA6400.024.9741125
TTGGGAG32300.024.8867846
GAGTAGC13800.024.868114
TAGGCGG1351.4559733E-524.37565
GGGAGGC36100.024.3470258
CGAGTAG6000.024.288993
AGTAGCT23550.023.9541855
CCCGAAT3550.023.8382851
TCGCGGG1000.001757100623.5050455
GGCGGAG8750.023.1021025
Produced by FastQC (version 0.11.5)