FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605828
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6550.044.484523
CCGAGTA7550.041.099722
CCCGAGT9500.037.1346781
CTACTCG5700.034.6430662
GAGTAGC17250.034.3273244
AGTAGCT27400.033.4458735
GCTGGGA58800.032.769149
CCCAAAG43050.032.6693151
GCTACTC14000.032.59011
GTAGCTG29400.032.129746
TACTCGG6450.032.059113
AAGTGCT33800.031.701275
TAGCTGG32500.031.5232947
AAAGTGC34500.031.0580544
TTGGGAG40450.030.904496
GGAGGCT44550.030.3810849
AGTGCTG37650.029.4581556
CCAAAGT41350.028.8801922
CGGAGGT9800.028.7729027
GTGCTGG38400.028.1484937
Produced by FastQC (version 0.11.5)