FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094189
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG1550.0031701159189.72217340-345
ACGGACT6103.0928903E-584.59155320-329
AGTAGCT73950.068.941135
CGAGTAG20250.065.690543
GTAGCTG80600.064.63486
GAGTAGC49600.064.3661354
GCTGGGA160150.062.238349
TAGCTGG86700.062.156777
AAGTGCT78300.061.7923055
CCGAGTA22050.060.9158132
TTGGGAG103100.056.2902956
CTACTCG17300.054.742452
AGTGCTG87950.054.238536
CCAAAGT92950.054.140232
AAAGTGC88600.053.840624
GCTACTC42300.051.8307651
CCCAAAG108200.051.630221
TACTCGG18400.051.447353
CAAGTAG31700.050.9413763
GGAGGCT116050.050.86199
Produced by FastQC (version 0.11.5)