FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094189
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA1050.0040592914174.69696245
CGCGATA656.319357E-573.281975105-109
AGTAGCT70100.073.164535
TTTCGCG1050.001894225172.89517210-214
CTACTCG20250.072.282392
CGAGTAG18000.069.840273
TACTCGG20800.069.802443
GAGTAGC45100.068.965654
GTAGCTG75200.068.670146
CCGAGTA19400.067.235152
CACGAAA6853.691337E-666.94591245
TAGCTGG80750.066.36017
AAGTGCT85450.066.168655
GCTGGGA171800.062.965559
GCTACTC47050.062.3471571
TTGGGAG116800.059.623426
CGCAAAA4650.004325185459.171547245
AAAGTGC94650.058.8640564
AGTGCTG94900.058.643966
CCAAAGT100450.058.638982
Produced by FastQC (version 0.11.5)