FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227242
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5750.035.1434753
CCGAGTA7650.034.4255032
CTACTCG5550.033.0464942
CGAGTAG7800.032.5344353
CGTACCG600.004164609631.3294533
CCCGAGT10150.030.601411
GCTGGGA64150.028.6433779
CGGAGGT11050.028.4942117
TAGCTGG34350.027.9092087
AGTAGCT32200.027.1457085
GCTACTC16650.027.134421
TTGGGAG45500.026.9571026
GAGTAGC20450.026.8866464
TAGGCGG1755.5697456E-926.8538197
GGAGGCT48300.026.6592279
GTAGCTG31950.026.4755956
CCCAAAG49200.025.9219551
GGAGGTT30400.025.0429528
GGGAGGC55050.024.926988
CTCGGGA9600.023.9866125
Produced by FastQC (version 0.11.5)