FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227242
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7250.035.0108152
TACTCGG7250.035.0102733
TTGGGAG47850.033.3982166
GCTGGGA65650.032.934379
GAGTAGC18450.032.864964
TAGCTGG31650.032.8204737
CGGAGGT11250.031.3353867
GTAGCTG31650.031.3353826
GGAGGCT55850.031.3073319
CTCGGGA9500.030.1818585
AGTAGCT32200.029.9252455
TCGGGAG9900.029.9110496
CCCAAAG52700.029.4376831
GGGAGGC62750.029.2130668
CCGAGTA7300.028.9758472
GGAGGTT34850.027.9186128
CGAGTAG6950.027.7292923
AAAGTGC41900.027.3725684
CGTGTCG1555.0838935E-827.2933772
TGGGAGG66200.026.9806187
Produced by FastQC (version 0.11.5)