FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254218
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1305.456968E-1239.815371
CGATACG804.7697133E-429.3887352
TTCGGCG650.006158844628.920155
CGGAGGT7450.026.4939637
GGAGGTT24750.023.5450948
TACTCGG5700.023.0853823
CTCGGGA6800.022.8065155
CGGCCTA1903.647583E-722.2609068
CCTATAG10250.022.0352921
CTCGCGT1303.16353E-421.6901134
TCGGGAG7000.021.4835416
CCGAGTA7450.021.4596942
GCTGGGA51050.021.357299
CGAGTAG7050.021.3311753
GGGAGGC40700.021.1305398
CTACTCG5600.020.9919532
CCCGAGT9700.020.8592221
TAGCTGG30000.020.8345587
TTGGGAG41650.020.7614046
GGCGGAG8850.020.7097685
Produced by FastQC (version 0.11.5)