FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512022
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG4550.036.1586883
CTACTCG5250.033.1289022
TAGCGGC953.7516184E-529.6870027
CCGAGTA5800.029.17692
CGAGTAG5050.028.8553493
CGGAGGT6750.028.5508337
GCTACTC12100.028.360861
GCTGGGA39750.028.1435269
CTCGGGA6300.026.8602035
AGGCGGA8000.025.8529454
GAGTAGC13050.025.5737954
TAGCTGG23650.025.0425247
GCGGAGG7700.025.0288286
AGTAGCT22350.024.8171445
CGCGGGT950.001304300624.7391687
GGGAGGC37800.024.4970118
CCCAAAG36000.024.4186151
GGAGGCT36400.024.4061419
GGCGGAG8200.024.0759145
GTAGCTG24250.023.6480566
Produced by FastQC (version 0.11.5)