FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638438
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG702.1732433E-433.604291
GCCGGTA1151.1393058E-732.6923078
CCGATAG953.7291164E-529.7132681
CGGAGGT6450.027.6870867
GCTGGGA43000.027.322789
CCGAGTA5650.025.797682
AGTAGCT22000.025.2065095
GTCGACT1501.1645261E-625.06415
TAGCGGG1501.1645261E-625.06416
CCCAAAG38900.024.7928831
CCCGTTA1351.4473615E-524.3942261
TACTCGG4250.024.326923
TAGCTGG25550.024.2793147
CGGCCTA1751.649496E-724.1689538
CCCGAGT7250.024.0096841
GCTACTC11200.023.5230031
CCTATAG7400.023.5230031
CGAGTAG5400.023.4975953
TAGTCCG1201.8402014E-423.4975956
CTACTCG4700.023.0089822
Produced by FastQC (version 0.11.5)