FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638438
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA550.002716899834.18372794
TACTCGG5800.034.0389673
CTACTCG5350.033.3875732
GCTGGGA47450.031.5013739
GAGTAGC13850.030.8849334
TAGCTGG26400.030.6240837
CGGAGGT8950.030.4609577
GGCGGAG10750.030.1714135
GGGAGGC45450.030.0952328
AGTAGCT24750.030.007995
TCGCCGT2550.029.49184294
TCGGGAG7500.029.4566546
TTCGGCG804.778857E-429.378875
TTGGGAG39050.029.3707896
GTCGCCG2800.028.53730894
GCTACTC13350.028.5222321
GGAGGCT38950.028.3595289
AGGCGGA11200.028.1197724
CGAGTAG5200.028.022923
CTCGGGA7400.027.9496255
Produced by FastQC (version 0.11.5)