FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394492
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6250.043.611453
CCGAGTA6950.041.2673072
CGTACCG608.832378E-539.162583
GCTGGGA59100.036.8963179
CTACTCG4800.036.2427942
GAGTAGC17500.035.447734
TACTCGG4850.034.8829573
TAGCTGG31300.034.382997
AGTAGCT26850.034.1305125
AAAGTGC34050.034.090424
CCCAAAG43250.033.8325651
CCAAAGT38150.032.906212
GTAGCTG28750.032.5287786
AAGTGCT36600.032.2288785
AGTGCTG38550.031.9395986
GCTACTC12900.031.731471
CGCGTTA300.00416313631.33202480-81
GTGCTGG39800.031.2907037
CGGAGGT10000.031.0167627
TTGGGAG38550.030.4767156
Produced by FastQC (version 0.11.5)