FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8050.049.6212583
CCGAGTA8950.044.6453742
CCCGAGT10400.041.6171
CTACTCG6850.041.1757242
TACTCGG7100.040.3753433
AGTAGCT32350.039.3676725
GAGTAGC21200.038.3490834
TAGCTGG36450.038.162687
GCTGGGA71900.037.909139
GTAGCTG35150.036.0986
CCCAAAG51600.035.283981
GCTACTC17750.034.455721
CCAAAGT47900.033.2693822
AAAGTGC41150.033.2328724
CGTCGAG851.7441214E-533.2084541
TTGGGAG45500.032.9476176
AAGTGCT41250.032.5826845
CGGAGGT11050.031.4712627
CGAACGC753.2790593E-431.3295023
TCGGGAG10850.031.1851256
Produced by FastQC (version 0.11.5)