FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620030
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG4900.045.0905233
CTACTCG4800.044.072032
CGAGTAG4250.033.1830143
GCTACTC13300.032.8730241
CGATACG753.2725246E-431.3401092
CCGAGTA5100.030.4183442
CGGAGGT8400.029.6572157
TTGGGAG36350.028.7077316
TAGTCCG1154.1951407E-628.612156
GGAGGCT38150.028.091989
GGGAGGC43050.027.7333668
CGACGGT856.814379E-427.6498729
ACTCGGG8050.026.862444
GGCGGAG10400.026.6682115
AGGCGGA10450.026.5411174
CCCGAGT6750.026.4659921
GCTGGGA43750.026.2152399
CTCGGGA8550.025.8408585
CCGTTAG1101.0173271E-425.6419092
CCCAAAG37550.025.2900541
Produced by FastQC (version 0.11.5)