FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810056
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5850.037.7567943
CTACTCG5200.036.180382
TACTCGG5600.036.0855673
GCTGGGA57300.033.5445379
CCCAAAG48200.032.5144771
GAGTAGC15200.032.1545834
CCGAGTA7200.032.0095862
CCCGAGT8850.030.3117561
AGTAGCT27500.030.2477935
TAGCTGG30050.029.5576887
CGGAGGT8350.029.2664457
TTGGGAG39150.029.1694096
AAAGTGC37550.029.1607784
GTAGCTG27750.028.7898276
CCAAAGT43600.028.263852
GGAGGCT41050.028.1627529
GTGCTGG40900.028.1511347
AGTGCTG41250.027.7983516
GAGGTTG32700.027.162349
AAGTGCT41100.026.9850545
Produced by FastQC (version 0.11.5)