FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800923
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA352.1871815E-433.56854272-73
GCGTATC600.004163742531.3306436
CGAGTAG7150.030.2351653
CGTCGAG1452.6302587E-829.209541
CCGAGTA7600.028.4627112
TACGCGA350.00884220826.85507888-89
TTCGGCG1057.429655E-526.8548375
GCTGGGA48350.025.4662729
CCCGAGT9850.025.3215241
CGCGCCC3550.025.1684952
GAGTAGC16950.024.9536114
TTGGGAG34500.024.7920746
AGTAGCT26100.024.668385
GTAGCTG25850.024.5433486
CGGAGGT8450.024.4712727
ACCGCGC2351.9826984E-1024.0305421
TAGCTGG28650.023.9490787
CCCAAAG41050.023.2719671
CGGGTAG1452.5366358E-522.6877087
GCTACTC13300.022.6453231
Produced by FastQC (version 0.11.5)