FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800923
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5400.035.6916123
TACTCGG5400.035.6916123
CCGAGTA6100.035.4502682
CTACTCG5900.034.2616272
TCGCGTT702.1839487E-433.5762755
GCTGGGA54300.032.8065959
CCCGAGT7900.032.7291641
CCCGTTA1201.6503509E-731.3406541
GCTACTC15350.030.3197881
TTGGGAG39550.029.949626
GTGCTGG33500.029.6046147
AGTAGCT26100.029.5368295
CGGAGGT9400.029.5016257
ACTCGCG804.777579E-429.380293
TAGCTGG29000.028.6877887
GGAGGCT43900.028.2657939
CCCAAAG44500.028.206591
GAGTAGC15350.028.1739524
CTCGCGT856.81209E-427.6515434
GTAGCTG27950.027.0758026
Produced by FastQC (version 0.11.5)