FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759227
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1055.064976E-835.850861
TCGCGTT600.004164338631.329715
CATCGCG700.008843115526.8540343
TACTCGG4650.025.2658943
CCGATAG950.001296304224.7653941
CCCAAAG36600.024.5556341
CTACTCG4450.024.3029772
CCGAGTA5900.023.9089722
CGAGTAG6100.023.8824833
GCTGGGA43150.023.8512389
GCGGGTA1602.0425814E-623.4972828
GGGAGGC34350.022.7106198
CGGAGGT5800.022.6870337
GGAGGCT31950.022.6515259
CTAGCGG1050.002337888622.3783656
TAGCGGT1703.4582063E-622.115097
GAGTAGC15400.021.6663254
AGCGCAC3101.2732926E-1121.2233529
TAGCTGG24650.020.3992637
CTCGTAT18400.020.17737894
Produced by FastQC (version 0.11.5)