FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525666
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1450.045.443061
TCGCTCG500.001698632837.617022
GTCGGTA804.7864515E-429.370845
GCTAACG1151.3647803E-424.5561871
TACTCGG4600.024.5182693
CTCGGGA5800.022.6864455
CTACTCG4450.022.1898142
TATAGCG1754.44716E-621.4826744
TCGGGAG6250.021.053026
GCTACTC12300.021.0457821
CCGAGTA5900.019.9242692
CGAGTAG5250.019.6924513
GCGAGTA1200.00502482319.6108441
TAGTCGG1200.005069926419.580563
GCCGTCT7300.019.31233494
GCTGGGA36300.019.1598229
CGGAGGT5900.019.1159387
GCGGAGG6400.019.0910476
CGTCGGG1508.302068E-418.797343
GTCGAGA3558.913048E-1118.5612491
Produced by FastQC (version 0.11.5)