FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525666
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT5400.033.078647
CTACTCG5050.029.7874682
CGCCGTA953.7515158E-529.68713294
GCTACTC12300.026.7527181
TCGCCGT2800.025.1810594
TACTCGG5650.024.9596923
AGTAGCT19150.024.5464675
GGGAGGC33850.024.162868
CGTGTCG1000.001757442723.5041732
CGAGTAG4450.023.2396223
GCTGGGA40050.023.239169
GGAGGCT32400.022.9229169
GCGGAGG7400.022.868026
GGCGGAG7850.022.754735
TTGGGAG29100.022.453276
CCGGGTT4700.022.0030368
AGGCGGA8650.021.7371044
TGGGAGG35400.021.644237
GAGTAGC12400.021.607824
CCGAGTA4700.021.0037292
Produced by FastQC (version 0.11.5)