FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483039
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4650.033.365122
TACTCGG5000.031.9679163
CTCGGGA5750.028.6157845
CCCGTAG1258.005869E-626.3297041
GCGGGCT3450.025.8878639
CGAGTAG4550.025.8305743
CGGAGGT6850.024.7043617
GCTGGGA36750.024.5587129
GAGTAGC10350.024.5278154
GCTACTC12300.024.4643021
GGAGGCT32650.023.8993789
ACGTCCG1000.001756717923.5058235
CCGAGTA5050.023.2744962
TAGCTGG22200.022.444747
ATAGCGG2103.8495273E-822.3864975
AGTAGCT21100.022.28045
GGGAGGC35350.021.8080148
CCCAAAG32800.021.7885171
ACTCGGG7650.021.5085964
TCGGGAG7250.021.397116
Produced by FastQC (version 0.11.5)