FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140915
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG2300.004982904163.08061310-319
CTAAACG2503.7250796E-4161.71196320-329
CGGTTAC4000.001112674683.321625250-259
AGACCGG5250.00935794977.00569320-329
CGAATCC4750.001656122275.376015340-345
ACTCGTA2600.00223733769.88873340-345
CACTCGT3900.003674383761.661324340-345
TAAACGG3503.201477E-457.75427320-329
GTTCGAC2100.005414596355.918694210-219
CGAGTAG13750.055.602293
CGATCTA1750.00592942154.6513310-319
AGTAGCT49100.052.0579535
GTAGACG3703.2776825E-550.687214310-319
TGTTCGA4700.00857448749.774193210-219
CCGAGTA15250.049.4156342
GAGTAGC34650.048.4099544
GCTGGGA105400.047.743979
TAGCTGG55200.046.821977
GTAGCTG53000.046.7201736
TTCGGAC5100.00738011546.66225340-345
Produced by FastQC (version 0.11.5)