FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619839
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG757.38732E-637.6442951
CGGCCTA1807.2759576E-1231.3301338
TTGGGAG35250.028.7970586
CCGAGTA4900.026.8692722
TACTCGG4550.026.85443
GCTGGGA44550.026.2666789
CCCAAAG39750.026.0432241
CGGAGGT7400.026.037887
ACCGCGC2208.731149E-1125.6665651
GGCGGAG8000.025.2599185
CCCGAGT7200.024.834781
GGAGGTT19350.024.2869268
CGAGTAG4850.024.224333
CCGGTTA1751.6494778E-724.1689599
GGGAGGC40700.023.67088
ATACCGG1602.042274E-623.49764
GGAGGCT35200.023.2305819
CCTTTAG16450.023.1701221
CCCGTAG2453.3469405E-1023.047531
CTACTCG4600.022.4884132
Produced by FastQC (version 0.11.5)