FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619839
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5100.037.7880133
CTACTCG5050.037.231372
CGGAGGT9150.036.9858137
GGCGGAG12050.035.497255
CGAGTAG5200.035.253453
AGGCGGA12200.032.7491074
GCGGAGG11100.032.6061746
CTCGGGA7100.032.4397965
CCGAGTA5850.032.1398962
CCCAAAG38950.030.89451
GGAGGTT24600.030.3798628
GCTGGGA49550.030.2601329
CTACCGT1401.8912033E-830.2172432
TCGGGAG8050.029.1948476
GGAGGCT42000.028.8731549
TTGGGAG38700.028.2993876
GAGTAGC13350.028.1675514
GAGGTTG29400.027.3383679
CCCGAGT6750.027.1587311
TAGGCGG1755.5551936E-926.8597745
Produced by FastQC (version 0.11.5)