FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985057
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT62150.066.558995
GACGTAT2251.04257546E-466.22625290-299
GTAGCTG66650.062.1558726
AAGTGCT73250.061.1789975
CGAGTAG18350.060.9715773
GAGTAGC42300.060.9061284
TAGCTGG71350.060.0110247
CCGAGTA19450.059.411822
GCTGGGA142200.059.201329
GATCGGT2500.007120867358.540565330-339
CTACTCG16350.056.245132
AAAGTGC79750.055.1309474
TTGGGAG85100.054.22346
CCAAAGT87500.053.724462
TACTCGG17250.053.640663
AGTGCTG81000.053.6081966
GCTACTC38550.052.6102941
CCCAAAG102250.051.156491
CAAAGTG88400.050.4888953
TATCGAC3157.4578566E-949.89931340-345
Produced by FastQC (version 0.11.5)