FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089994
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA1550.048.568521
CGTCGAG2450.030.727021
CGAGTAG7250.022.6865883
CCGAGTA7750.021.8406182
GCTGGGA59700.020.3088139
GAGTAGC18450.019.103114
TACTCGG6150.018.3389843
CTACTCG5900.018.3290482
GTCGAGA4500.017.7623522
AGTAGCT29500.017.5230565
GACGTCG2002.969826E-416.4677641
TAGCTGG33950.016.333587
CCCGAGT12100.016.3316651
CCCAAAG49650.016.2995551
GTAGCTG31800.016.2556656
CGAGACT12200.016.19781
GTGCTGG43100.016.137037
TTGGGAG40150.016.0351926
AAGTGCT42500.015.9225535
CGCGTGG1800.002814046815.664554
Produced by FastQC (version 0.11.5)