FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089994
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7150.028.269582
TACTCGG8100.025.5343363
CGAGTAG7450.025.238333
CGCCGTA2950.023.90039694
AGTAGCT32400.023.7933585
CCGAGTA8350.023.6439322
GCTGGGA63800.023.5764929
GCTACTC18800.023.2550451
GTAGCTG33500.022.5907676
GAGTAGC20450.021.8367124
GCCGTAT2859.640644E-1121.4404794
CTCGGGA9350.021.1151715
TAGCTGG37400.020.2347187
CCCAAAG48400.019.9113451
TCGCCGT5500.018.80164594
AAAGTGC41700.018.7123784
AAGTGCT43000.018.4746065
GGAGGCT48200.018.1391039
CCAAAGT47400.018.0488682
TCGGGAG11000.017.9476056
Produced by FastQC (version 0.11.5)