FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017402
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5300.033.6939963
CTACTCG5400.032.216322
CCGAGTA6000.031.3456082
CGAGTAG5600.031.0497763
CGGAGGT8200.028.6550357
GCTGGGA52750.028.1520129
AGTAGCT26000.026.3890845
GCCGTAG2550.025.8350181
TAGCTGG29250.025.5455977
AACGCGG950.001306006224.733825
CCCGAGT9350.024.66071
GGCGGAG9850.024.3320545
CCCAAAG45400.024.1502131
CTCGGGA6550.023.6764975
GAGTAGC15050.023.4190654
GTAGCTG29300.023.4169356
GCTACTC16200.023.2378461
GGAGGTT27200.023.1515858
CCGGGTT4700.022.997198
TCGGGAG7100.022.5042926
Produced by FastQC (version 0.11.5)