FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017402
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG802.4572182E-741.1316643
CTACTCG5750.035.971642
TCGCGTT801.1581176E-535.2551275
TACTCGG6350.034.052763
CGGAGGT9400.033.0037087
GCTACTC15200.030.617311
CCGAGTA6400.030.1147542
GCTGGGA57150.029.198389
TTGGGAG44000.028.8451066
CTCGCGT1154.1946114E-628.6128564
CGAGTAG5950.028.4415843
GGGAGGC53450.027.877788
GGAGGCT45650.027.5956529
CCCAAAG47450.026.7487181
CCCGAGT9500.026.720561
AGTAGCT27800.026.7161185
GCGGAGG11700.026.5166786
TAACCGG1606.9141606E-826.4413456
GGAGGTT29750.026.2281558
GGCGGAG12400.025.3988555
Produced by FastQC (version 0.11.5)