FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012429
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG2150.002445935122.152954340-345
ACTCGTC3800.001301586580.09701310-319
TGCGCGG2750.001560475176.52916200-209
AACGCAC4902.9061994E-760.188736330-339
TGAGTAC13950.005185252556.479324340-345
TCGTATG9405.186453E-455.878483340-345
CCAACGC4700.007305076351.831795330-339
CGGGCAA3955.830366E-551.070175310-319
CTTACGG3700.050.345047300-309
AGTAGCT42600.043.7327275
CGAGTAG11450.043.055213
CCGAGTA12000.042.5311742
ACGGGTA4206.8552345E-641.942284340-345
GTAGCTG44450.041.3967326
GAGTAGC28150.041.338094
GCTGGGA90650.041.2299239
GGCATAG12856.176833E-740.876087340-345
TAGCTGG46100.040.0394137
CGCAACG1400.00391783639.85838320-329
CTACGGG4702.1732963E-539.633335300-309
Produced by FastQC (version 0.11.5)