FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372195
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG757.379722E-637.6497231
CTCGCGT1152.7812348E-936.7776874
ACTCGCG851.7571741E-533.1720283
TACTCGG3950.032.122283
CTACTCG4400.032.064822
CGCGTTG1052.0687494E-631.329146
TCGCGTT902.5974548E-531.3291365
CGTTCGG650.00615949828.9192054
CCCGTAG1853.237801E-1027.98291
CGGAGGT5400.025.237367
CGAGTAG4100.025.2161373
ACCGCGC1751.6268132E-724.2033941
GCTGGGA39150.024.0070049
CCCAAAG33200.023.5310781
GCTACTC13150.023.2626611
CCGAGTA4550.022.7390082
CTCGGGA5900.022.30215
GGAGGCT32150.022.0716959
CCGATAA1954.6193236E-721.7209931
CCGGGTT4450.021.120778
Produced by FastQC (version 0.11.5)